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Anyone shooting through a microscope?

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grsnovi

grsnovi

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I recently purchased an AmScope stereo trinocular scope and their DSLR adapter. I previously had a Vello A-mount-to-T-mount lens adapter and mounted my a65 to the scope. With everything tight, I can move the camera upwards (father away from the optics in the 2x adapter that fits into the third tube). One thing that I wasn't initially thinking about was the sensor plane relative to the subject and how that potentially impacts focus and DOF. My microscope has both zoom and focus on two different sets of knobs. I'm not sure if I should be trying to focus through the eye pieces or using the focus peaking on the camera screen?

Below is a shot through the scope as well as a shot of the scope with camera mounted. Both images have been scaled to 1000 pixels wide.



[ATTACH alt="6" rule showing approx. 4" from ring light to table top "]3133924[/ATTACH]
6" rule showing approx. 4" from ring light to table top



Synthetic ruby is 1.2mm in DIA
Synthetic ruby is 1.2mm in DIA
 

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I've occasionally done a very rudimentary setup.

Sony A7iii, Bresser microscope I got in Aldi years ago, cheapo LED ring light,
Sony A7iii, Bresser microscope I got in Aldi years ago, cheapo LED ring light,

Slightly unsavoury subject, but here's a head louse at x3 magnification, 9 images focus stacked.



1a18709848104117af35572260828808.jpg.png
 
Thank for that Ken! Looks like a lot of nature shots but I applied to register and will spend some time there.
 
My setup has a stand rather than pole mount.

47cd6557b9eb4a2cb5364d18992cfdee.jpg

4x zoom with 2x relay lens so typically 2x-8x.
Even more when I use extension tubes between the camera and the relay lens.

--
Bill ( Your trusted source for independent sensor data at PhotonsToPhotos )
 
When my eyes see sharp focus, my camera isn't focused on what I want. I wish that I had a micro-adjust between the camera and the scope. When the camera focus peaks, the shot isn't in focus. Tonight I started above focus and moved below focus and I suspect my DOF is less than .1mm. I'm not sure that I even have enough adjustment to get a meaningful sequence to focus stack.



37a885d2796c49e396071bf5b357a046.jpg
 
Focus stack? Just try it...good photography involves a lot of experimenting.
 
Gregor Samsa said:
I've occasionally done a very rudimentary setup.

Sony A7iii, Bresser microscope I got in Aldi years ago, cheapo LED ring light,
Sony A7iii, Bresser microscope I got in Aldi years ago, cheapo LED ring light,

Slightly unsavoury subject, but here's a head louse at x3 magnification, 9 images focus stacked.

1a18709848104117af35572260828808.jpg.png
Click to expand...
Aldi? The grocery store?
 
I had thought that I'd captured enough images yesterday to do a focus stack however when I finally looked at them the only image that was reasonably in focus was the one where I had focus peaking indications.

You're right - I need to experiment more.

I may also need to consider that shooting through my macro lens with an attached Raynox may ultimately prove to be a "better" solution for what I want/need. It's not like I'm shooting for publication.
 
Yes, Aldi, the German discount, no frills grocery chain. Not sure about how it works where you are, but here in Europe - or at least Ireland - they (and Lidl) are famous for their “middle isle” smorgasbord of random goods that change every week. Could be wetsuits this week, hedge trimmers next week, microscopes the week after.
 
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