light microscope is that for everyone and if so how do you do it?

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light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Changing the topic slightly, there is an exciting new type of microscope just coming out of Germany called a SeeNano microscope. Kind of exciting what it can do. For instance, how about 8000x, in color with no oil immersion? And a deep field of view too? And ability to see live virus without staining.

I have no idea what these scopes will sell for, but if you have to ask, then you aren't going to be able to afford one.

According to the site, the first models for sale will come out at the end of May and they already have over a year's manufacturing backlog of those wanting one.

I really don't know how these scopes work. But I suppose as they start getting used by places that can actually afford them, we should start hearing more about them.

SeeNano in Comparison - Grayfield Optical Inc - High Resolution Optical Microscopes

The site has numerous examples showing what this scope can do. I've always like microscopes, but I doubt I will ever get close to one of these scopes to actually see one.
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Changing the topic slightly, there is an exciting new type of microscope just coming out of Germany called a SeeNano microscope. Kind of exciting what it can do. For instance, how about 8000x, in color with no oil immersion? And a deep field of view too? And ability to see live virus without staining.

I have no idea what these scopes will sell for, but if you have to ask, then you aren't going to be able to afford one.

According to the site, the first models for sale will come out at the end of May and they already have over a year's manufacturing backlog of those wanting one.

I really don't know how these scopes work. But I suppose as they start getting used by places that can actually afford them, we should start hearing more about them.

SeeNano in Comparison - Grayfield Optical Inc - High Resolution Optical Microscopes

The site has numerous examples showing what this scope can do. I've always like microscopes, but I doubt I will ever get close to one of these scopes to actually see one.
Sounds all a bit weird. A microscope's useful magnification is limited by the wavelength of light and the numerical aperture of the objective lens in my playbook. That means around 1000x linear is about the useful limit. Empty magnification past that point.

Some reading that seems to agree with what I learned from books when I first bought my lab microscope. https://findanyanswer.com/what-is-the-highest-magnification-for-a-compound-light-microscope

If physics has changed since then I'd like to know.
 


If physics has changed since then I'd like to know.
Once I started digging around I found more quacks than facts but the better microscope does work. It seems to use some form of optical trickery with diffraction gratings or somesuch to modify the optical results plus some computer processing to get a result. Good luck with finding a clear description of how it works.

It's a case of "trust us, it's good. Pay up now to get in line to obtain one sometime in the future".

If it does work then it removes the need for the electron microscope to examine some small structures. The advantage being able to examine live specimens and not having to be killed and prepared as for electron microscopes, that preparation changes the shape and nature of many subjects. https://www.sciencelearn.org.nz/resources/500-preparing-samples-for-the-electron-microscope
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Changing the topic slightly, there is an exciting new type of microscope just coming out of Germany called a SeeNano microscope. Kind of exciting what it can do. For instance, how about 8000x, in color with no oil immersion? And a deep field of view too? And ability to see live virus without staining.
Yes, how about that? It's supposed to solve just about all the problems of microscopy. Where are the publications? It's a trade secret, it seems. But the revolutionary principle has been in use for decades, but was virtually a secret because everything was in German. Yeah, right.

Their explanation of the principle looks like gibberish to me, possibly designed to fool the public. And they charge for demonstrations.
 
If physics has changed since then I'd like to know.
Once I started digging around I found more quacks than facts but the better microscope does work. It seems to use some form of optical trickery with diffraction gratings or somesuch to modify the optical results plus some computer processing to get a result. Good luck with finding a clear description of how it works.

It's a case of "trust us, it's good. Pay up now to get in line to obtain one sometime in the future".

If it does work then it removes the need for the electron microscope to examine some small structures. The advantage being able to examine live specimens and not having to be killed and prepared as for electron microscopes, that preparation changes the shape and nature of many subjects. https://www.sciencelearn.org.nz/resources/500-preparing-samples-for-the-electron-microscope
Yes, I agree. I don't quite know how this new microscope actually works, but i would like to know.

There is yet an older microscope that could see live virus:

Royal Rife's Universal Prismatic Microscope (frequencyrising.com)

He used the physics law that when two frequencies are mixed together that a sum and difference freq are produced as well as the two originals. We have been using this for radio for over 100 years. His scope used uv light and quartz prisms and other tricks to turn the result into visual light. Many doubted him, and many made the trip to look through his scope and came away shocked!

Problem was that this scope was so complex to use, that only Royal Rife could make it work.
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Changing the topic slightly, there is an exciting new type of microscope just coming out of Germany called a SeeNano microscope. Kind of exciting what it can do. For instance, how about 8000x, in color with no oil immersion? And a deep field of view too? And ability to see live virus without staining.
Yes, how about that? It's supposed to solve just about all the problems of microscopy. Where are the publications? It's a trade secret, it seems. But the revolutionary principle has been in use for decades, but was virtually a secret because everything was in German. Yeah, right.

Their explanation of the principle looks like gibberish to me, possibly designed to fool the public. And they charge for demonstrations.
Well, if it works as advertised and they start shipping at the end of May, we should start hearing reports about how well (or bad) the product is. I will watch for developments, but only out of curiosity.
 
Just on the off chance someone reads this the other big issue with microscopy is preparation of the specimens. I did this a little as an undergraduate in 1976 and still bear the scars.
 
Just on the off chance someone reads this the other big issue with microscopy is preparation of the specimens. I did this a little as an undergraduate in 1976 and still bear the scars.
That's an important point!

I took my first photomicrograph , using a Swift monocular microscope of 1890 vintage coupled to a 1930's Box brownie. The subject was the algae Spirogyra in water from my garden pond. In 1949, I was eight years old and had quite a time learning how to use the scope. That was relatively easy with tiny pond life as the subject. Larger organisms proved MUCH more difficult, since I had to learn how to prepare them for microscopy! That started an odyssey lasting over fifty years. Throughout my career as a medical scientist, I continued to learn new microtechniques and never lost my love of photomicroscopy.

I still have my original scope together with several others and use them on a regular basis I am teaching my grand children pond life microscopy and so the cycle continues!

To the OP, It's not beyond your abilities, just try.
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Changing the topic slightly, there is an exciting new type of microscope just coming out of Germany called a SeeNano microscope. Kind of exciting what it can do. For instance, how about 8000x, in color with no oil immersion? And a deep field of view too? And ability to see live virus without staining.
Yes, how about that? It's supposed to solve just about all the problems of microscopy. Where are the publications? It's a trade secret, it seems. But the revolutionary principle has been in use for decades, but was virtually a secret because everything was in German. Yeah, right.

Their explanation of the principle looks like gibberish to me, possibly designed to fool the public. And they charge for demonstrations.
Well, if it works as advertised and they start shipping at the end of May, we should start hearing reports about how well (or bad) the product is. I will watch for developments, but only out of curiosity.
I shall look forward to it with popcorn, but I doubt if I'll read about it in the press.

By the way, guys, don't get any ideas. The equipment they showed pictures of was basically ridiculously expensive research equipment.
 
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light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
If I understand your question there is more than one way to do this.

I use a stereo zoom tri-nocular microscope.
I mount my tethered camera on the third tube and capture images for stacking directly to my computer.

For example:

1592a6a356634657b2cd4eb31a41dcc2.jpg
Not sure what the original poster is after.

I would just point out this this type of setup "only" goes to about 8x.
That's far less than traditional compound microscopes but more than enough to be interesting for me.
And, FWIW, specimen preparation is much easier.

Here's are a couple examples:

Hibiscus pollen
Hibiscus pollen

flower part
flower part

 Acetaminophen using cross polarized light.
Acetaminophen using cross polarized light.

--
Bill ( Your trusted source for independent sensor data at PhotonsToPhotos )
 
Many models to choose from. The one I have has a 10x-50x range and a 220x power.

USB Digital Microscope Cameras | Dino-Lite (dinolite.us)

And I have the stand that provides a focus knob.

Accessories: Stands & Parts (dinolite.us)

Not big sensors with lots of pixels, but can see things I can't see with my camera and macro lenses.

Here are a couple images taken with it. Original only 1280x1024 pixels. But it responds nicely to Gigapixel_AI and these have been resized X2.

e1326e7e76164264bde82f774a7d7bf1.jpg

Looked like some bacteria colonies on a dead rose leaf.

e252f0d13be348a3ae0eb4d0e9853d87.jpg
 
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Nice. Are these single frames or stacked?
They were single frames. But I can manually stack and process if desired.
 
If physics has changed since then I'd like to know.
Once I started digging around I found more quacks than facts but the better microscope does work. It seems to use some form of optical trickery with diffraction gratings or somesuch to modify the optical results plus some computer processing to get a result. Good luck with finding a clear description of how it works.

It's a case of "trust us, it's good. Pay up now to get in line to obtain one sometime in the future".

If it does work then it removes the need for the electron microscope to examine some small structures. The advantage being able to examine live specimens and not having to be killed and prepared as for electron microscopes, that preparation changes the shape and nature of many subjects. https://www.sciencelearn.org.nz/resources/500-preparing-samples-for-the-electron-microscope
Yes, I agree. I don't quite know how this new microscope actually works, but i would like to know.

There is yet an older microscope that could see live virus:

Royal Rife's Universal Prismatic Microscope (frequencyrising.com)

He used the physics law that when two frequencies are mixed together that a sum and difference freq are produced as well as the two originals. We have been using this for radio for over 100 years. His scope used uv light and quartz prisms and other tricks to turn the result into visual light. Many doubted him, and many made the trip to look through his scope and came away shocked!

Problem was that this scope was so complex to use, that only Royal Rife could make it work.
There's a lot about Rife, widely regarded as an alternative medicine quack. So even if he invented something real the world at large seriously doubted him. I have a relative in the alternative medicine business and am well aware of the snake oil being peddled by many of those "experts".

A great to and fro page here from years back https://sci.techniques.microscopy.narkive.com/fljNXsYM/ergonom-500 with doubters and believers involved.
 
The problem here is that we are seeing 2D representations. The actual view though a stereo microscope is something else entirely.

The very first one I looked through many years ago was very low magnification of a covered petri dish with a bunch of ladybirds munching on something tasty. Truly breathtaking to watch in 3D at what was probably about only about 4x or 5x life size.

I can get a bit of the same sort of thing with my Pentax Papilio binoculars which have close focus that allows an enlarged view of insects at a safe for them distance.
 
inexpensive 1000x usb microscope

a google searche for "usb microscope camera" hit on this...many inexpensive 1000x microscope using USB to send images to computer

https://www.amazon.com/USB-Microscopes/b?ie=UTF8&node=2742273011
Cheap and 1000x magnification are mutually exclusive facts.

To get to 1000x you usually need oil immersion objectives and a reliable mechanical design to hold everything steady and in alignment. The 100x objective plus the 10x eyepiece will cost $many alone. In Australia the cheapest 100x oil immersion objective I could find is AU$260 and the necessary 10x eyepiece is guess about AU$30 at the cheapest. Plus a microscope body to hold it all together. Think more like AU$1000 all up to get something reliable and good as a minimum to do reliable and convenient 1000x observations.

Naturally 1000x is limited in use and more for stained bacterial material or sliced tissue samples and the like, depth of field is measured in microns so only thin or flat things can be viewed and they need to be carefully prepared in a slide with a glass cover for the oil immersion objective to work. Looking at living pond life is more down in the 5x to 50x linear observation range, which is what I suspect that linked cheap device handles.

My guess the "1000x" in those cheapy "microscopes" refers to 1000x area magnification and not 1000x linear magnification, so the likely limit is maybe 32x linear magnification (square root of 1,000). Common marketing trick with cheap microscopes and magnifiers.

They are fun to play with but most people soon tire of their inadequacies, I even have a $10 or $15(?) clip-on for a smartphone or tablet that gives reasonable super macro results. There are better ones of course https://thedroidguy.com/macro-lens-for-smartphones-1089257
Nice idea but don't expect good image quality or reliability at those prices.


sure, they are what they are...

here's a review of one, comparing the "fun 1000x microscope" to a professional microscope, at '1000x'...




0dcb91de835d47a6b6fbdc8e3a9d6234.jpg.png
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Found this youtube showing a very interesting technique to improve what is seen by a microscope.

We Upgraded Our Microscope! - YouTube
Thanks, I keep learning.

The DIC microscopes seem to be doing what that Grayfield people are doing but with less complication and secrecy plus clear explanations at how DIC works from various sites, here's Olympus https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/dic/dicoverview/ and https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/dic/dicintro/

Nice idea if you have a spare $12,000 or more to spend. https://www.spachoptics.com/E600-p/nikon-eclipse-e600-dic.htm and https://microscopecentral.com/products/olympus-bx41-dic-microscope
 
Just on the off chance someone reads this the other big issue with microscopy is preparation of the specimens. I did this a little as an undergraduate in 1976 and still bear the scars.
That's an important point!

I took my first photomicrograph , using a Swift monocular microscope of 1890 vintage coupled to a 1930's Box brownie. The subject was the algae Spirogyra in water from my garden pond. In 1949, I was eight years old and had quite a time learning how to use the scope. That was relatively easy with tiny pond life as the subject. Larger organisms proved MUCH more difficult, since I had to learn how to prepare them for microscopy! That started an odyssey lasting over fifty years. Throughout my career as a medical scientist, I continued to learn new microtechniques and never lost my love of photomicroscopy.

I still have my original scope together with several others and use them on a regular basis I am teaching my grand children pond life microscopy and so the cycle continues!

To the OP, It's not beyond your abilities, just try.
Back about age 15 and following years I spent many hours peering at pond life, always fascinating. Low magnification and dark field illumination made for less eye strain. No mechanical stage, I simply slid the slide about by hand to follow some of the more frisky swimmers. For the uninitiated in the compound microscope everything is upside down and flipped left to right, so learning to move things the correct way soon becomes second nature.

For me microscopy was always a hobby, just like photography is for me.
 
light microscope is that for everyone and if so how do you do it?

No idea how it is done and how complex it is. Never seen it mentioned except jsut recently.
Found this youtube showing a very interesting technique to improve what is seen by a microscope.

We Upgraded Our Microscope! - YouTube
Thanks, I keep learning.

The DIC microscopes seem to be doing what that Grayfield people are doing
I doubt it.
DIC is a well known technique.
Yes, they've gotten cheap.
 
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