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Hmm, you raise an interesting point. In terms of sharpness and disregarding DoF for now, it better to shoot wide open at a lower ISO and f1.8 aperture or to stop down slightly to f2 or f2.8 and use a higher ISO?As others have said, larger sensor (yay NEX!), higher ISO, wider (lower) aperture, faster (lower aperture) lens. Also, don't zoom--it kills your aperture.
I find with the NEX that I don't have to go higher than ISO 1600 for kids if I have a fast f1.8 lens.