Re: New Focus Stacking Tables
1
Richard Turton wrote:
The previously posted Focus Bracketing table has been reworked for the Focus Stacking function of the E-M1. The differences between Focus Stacking and Focus bracketing are:
1. With Focus Stacking the final output is produced in camera as a JPEG. With Focus Bracketing the shots have to be combined later using third party software.
2. The Number of Shots is fixed at 8 and not adjustable.
3. The initial focus point is located part way through the sequence instead of at the beginning. For Focus Stacking the step sequence is 0, -2, -1, 1, 2. 3. 4. 5 where 0 is the initial focus point. For Focus Bracketing the step sequence is 0, 1, 2, 3, 4, 5, 6, 7.
Separate tables are provided for each aperture. The total depth of focus for 8 shots is shown as a function of Focus Distance (runs vertically) and Focus Differential (runs horizontally). If the Focus Differential is greater than 3 there is a risk that the depth of field may be less than the focus step. Depth of field is not considered here. The original document is in PDF, which has been converted to JPEG so that it can be embedded here. It should fit on a single letter size sheet.

I just tested these out in the range of 200mm with the 60mm lens with settings from F5.6, 8, 11 focus step 5, f8 and F11 step 4, and F8 and F11 step 3. I found results very similar to your chart results in that range, perhaps 1.5mm less total DOF on average - but that is totally dependent on the limits of my eyesight and measuring methodology. I set up at an approximate down angle of 45 degrees at minimum focus distance +- (I measured with a tape measure) and shot a ruler with a $20 bill beneath it. I focused at the 3" mark on the ruler but adjacent to the bill such that focus peaking showed equal amplification on either side of the edge of the ruler adjacent to the bill. I felt that my vision with the marks on the ruler would not have allowed accurate measurement but that the ink patterns on the bill allowed me to be accurate to around 1/32" or .8mm.

My interest at the present time is shooting mosses, not so much for photographic artistry but as to be able to firmly identify most mosses primarily from the general habit or growth form of the mosses, their habitat, and in focus stacking with the macro lens to obtain leaf detail that is on the order of 3X more magnified than a 15X loupe.
I originally started out with single, manually focused images at F11. All of the macro subjects require a tripod and I used a 12 second self timer to minimize my effect on the steadiness of the camera. Mosses are, however, very difficult subjects in that some are on the ground, some on branches, and some on various parts of tree trunks. Of necessity the tripod is often placed in difficult positions on sometimes not too stable of a substrate. Nonetheless, it was generally possible to get good images with that DOF but sometimes critically visible leaves (not very many are completely visible) were not in focus and besides the images looked better with even more DOF. Thus I tried focus stacking and read yours and Lester's threads to get a rough handle on the appropriate aperture and focus stack step given my estimate that on average I needed about 1/2 to 3/4" to be in focus. With my initial tests of focus stacking in the field I chose F11, step 5. I found, of course, that I was unable to use a self timer with focus stacking employed so in reviewing some of my initial sets of images I noticed that the first one or two images sometimes were not sharp, suggesting I moved the camera on pressing the shutter. Interestingly, some of these stacks still turned out OK though I suspect they were not up to the best quality I could obtain.
I have since discovered that I can set a 2 second delay in silent mode which is the mode of focus stacking and future results should be better. My test above and your chart indicate that my initial choice of parameters for focus stacking were close and that I should be able to get the results I would like by shooting at F8 with a focus step of 5. It may well be that with some practice I may be able to recognize situations that would benefit from somewhat different settings. As you indicated in your last posts on this thread, there is a practical limit to how far the mathematics of a particular shooting situation can be helpful. Mosses, for example, unlike my tests (and yours and Lester's) are not strictly planar but also rise in the vertical. It would take a ton of trigonometry and measurement to be able to precisely quantify settings in advance. Here is an example of one of my earlier focus stacks. The curvature and tips of the leaves Identifies the species.

Claopodium crispifolium
Anyway, thanks again for your and Lester's works on focus bracketing and focus stacking. Thanks to your enlightenment, I will probably also learn to use my 8mmFE for situations in which I want to shoot wildflowers very close with a DOF to infinity.