Processing Raw images for scientific use

Started 11 months ago | Questions thread
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amirber New Member • Posts: 18
Processing Raw images for scientific use

Hello,

We have recently purchased a Sony a7R IV camera and intend to use it for scientific data analysis. For this reason we collect image in manual mode and only use the raw images. the processing is rather straightforward:
* capture dark image (with closed lens cap in a dark room)
* capture an image of the test object.

While we were experimenting with different settings of the camera I made sure to collect dark images under the same mode, ISO and exposure time and got very consistent values there, however when I processed the target images parts of the frame (about 1%) of the pixels were of lower values than those of the dark images. This came as somewhat of a surprise to me, is it possible that some unaccounted parameter caused the black level / pedestal to change automatically?

I process the raw images using a standard python library (rawpy) and would like to verify if I'm missing something in the process. If there is a whitepaper relevant for this I would very much appreciate it if you could point me in the right direction. I usually use computer vision cameras (Basler) which use Sony sensors, This is not an issue there so I'm guessing theres some free parameter hiding from me.

Amir

ANSWER:
Sony a7R
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