Hardest shot I ever took

Challenge #61 in the Eclectic series. Hosted by Ro13.
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Ever had one of those shots that just took forever to get it right? No editing...just the perfected shot, and maybe a description of what made it hard.
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little bee
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Submitted: Sunday, 20th March, 2011 16:19 (GMT)
Taken: Friday, 19th March, 2010
Focal length: 100 mm
Shutter speed: 1/1500 sec
Aperture: F4
ISO: 800
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