Hardest shot I ever took

Challenge #61 in the Eclectic series. Hosted by Ro13.
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Ever had one of those shots that just took forever to get it right? No editing...just the perfected shot, and maybe a description of what made it hard.
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Rana arvalis...
by Janolus
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Submitted: Sunday, 20th March, 2011 12:30 (GMT)
Taken: Sunday, 28th March, 2010
Focal length: 200 mm
Shutter speed: 1/320 sec
Aperture: F2.8
ISO: 200
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